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n1 s1 rat hcc cell line (ATCC)

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ATCC n1 s1 rat hcc cell line
N1 S1 Rat Hcc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+hcc+cell+lines/pmc11443676-58-4-12?v=ATCC
Average 95 stars, based on 156 article reviews

n1 s1 rat hcc cell line - by Bioz Stars, 2026-08

95/100 stars

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Animal experimental design. (A) Measurement of the baseline metabolism in N1S1 tumors, McA-RH7777 tumors, and normal liver tissues. (B) Measurement of the metabolism level in nonstimulated tumors (NS_T) and stimulated tumors (S_T) in both N1S1 and McA-RH7777 models. Red arrows demarcate the time of tumor and liver tissue harvesting. 1H MRI = proton MRI, HP 13C MRI = hyperpolarized 13C MRI, MS = mass spectrometry, RFA = radiofrequency ablation.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Animal experimental design. (A) Measurement of the baseline metabolism in N1S1 tumors, McA-RH7777 tumors, and normal liver tissues. (B) Measurement of the metabolism level in nonstimulated tumors (NS_T) and stimulated tumors (S_T) in both N1S1 and McA-RH7777 models. Red arrows demarcate the time of tumor and liver tissue harvesting. 1H MRI = proton MRI, HP 13C MRI = hyperpolarized 13C MRI, MS = mass spectrometry, RFA = radiofrequency ablation.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: Mass Spectrometry

N1S1 and McA-RH7777 hepatocellular carcinoma (HCC) subtypes exhibit different degrees of lactate flux. (A) Hyperpolarized (HP) carbon 13 (13C) pyruvate and HP 13C lactate spectroscopic signals over time and representative images of 13C-lactate MRI (green) superimposed on T2-weighted 1H images (grayscale) in both N1S1 and McA-RH7777 tumors. Tumor outlined in dotted red; adjacent normal liver outlined in dotted white. Blue arrow: kidney; yellow arrow: spine; green arrow: stomach. 13C lactate signal intensity scaling was adjusted between the N1S1 and McA-RH7777 images to allow visualization of the lactate signals in the latter. (B) Lactate flux, measured as the lactate-to-pyruvate ratio (LPR), in tumor (T) and adjacent normal liver (NL) in both HCC subtypes. (C) Baseline lactate flux as expressed in kPL for N1S1 (N) and McA-RH7777 (M) tumors, also showing greater flux in N1S1. Error bars represent SD.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: N1S1 and McA-RH7777 hepatocellular carcinoma (HCC) subtypes exhibit different degrees of lactate flux. (A) Hyperpolarized (HP) carbon 13 (13C) pyruvate and HP 13C lactate spectroscopic signals over time and representative images of 13C-lactate MRI (green) superimposed on T2-weighted 1H images (grayscale) in both N1S1 and McA-RH7777 tumors. Tumor outlined in dotted red; adjacent normal liver outlined in dotted white. Blue arrow: kidney; yellow arrow: spine; green arrow: stomach. 13C lactate signal intensity scaling was adjusted between the N1S1 and McA-RH7777 images to allow visualization of the lactate signals in the latter. (B) Lactate flux, measured as the lactate-to-pyruvate ratio (LPR), in tumor (T) and adjacent normal liver (NL) in both HCC subtypes. (C) Baseline lactate flux as expressed in kPL for N1S1 (N) and McA-RH7777 (M) tumors, also showing greater flux in N1S1. Error bars represent SD.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques:

Metabolic profiles of N1S1 and McA-RH7777 hepatocellular carcinoma (HCC). (A) Multivariable analysis of metabolomic data using the partial least-squares discriminant model. (B, C) Altered metabolites (upregulated, B; downregulated, C) in N1S1 (N) and McA-RH7777 (M) relative to normal liver. (D) Normalized lactate levels in normal liver from control rats (C_NL), McA-RH7777 tumor (M_T), and N1S1 tumor (N_T). Error bars represent SD. F1,6BP = fructose 1,6-bisphosphate; F6P = fructose 6-phosphate; G3P = glyceraldehyde 3-phosphate; G6P = glucose 6-phosphate; NADPH = nicotinamide adenine dinucleotide phosphate; R5P = ribose 5-phosphate.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Metabolic profiles of N1S1 and McA-RH7777 hepatocellular carcinoma (HCC). (A) Multivariable analysis of metabolomic data using the partial least-squares discriminant model. (B, C) Altered metabolites (upregulated, B; downregulated, C) in N1S1 (N) and McA-RH7777 (M) relative to normal liver. (D) Normalized lactate levels in normal liver from control rats (C_NL), McA-RH7777 tumor (M_T), and N1S1 tumor (N_T). Error bars represent SD. F1,6BP = fructose 1,6-bisphosphate; F6P = fructose 6-phosphate; G3P = glyceraldehyde 3-phosphate; G6P = glucose 6-phosphate; NADPH = nicotinamide adenine dinucleotide phosphate; R5P = ribose 5-phosphate.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Enriched Metabolic Pathways in Tumors from N1S1 and McA-RH7777 Rat Hepatocellular Carcinoma Cell Lines Compared with Normal Liver from Control Rats

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Enriched Metabolic Pathways in Tumors from N1S1 and McA-RH7777 Rat Hepatocellular Carcinoma Cell Lines Compared with Normal Liver from Control Rats

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: Control

Expression of metabolites relating to glycolysis and the pentose phosphate pathway (PPP) in normal liver from control rats and tumors from N1S1 and McA-RH7777. Each graph shows the metabolite expression in normal liver (C_NL, left bar), McA-RH7777 (M_T, middle bar), and N1S1 (N_T, right bar). Error bars represent SD. 1,3-BPG = 1,3-bisphosphoglyceric acid; 3PG = 3-phosphoglyceric acid; 6-PG = 6-phosphogluconic acid; 6-PGL = 6-phosphogluconolactone; DHAP = dihydroxyacetone phosphate; E4P = erythrose 4-phosphate; F1,6BP = fructose 1,6-bisphosphate; F2,6BP = fructose 2,6-bisphosphate; F6P = fructose 6-phosphate; G3P = glyceraldehyde 3-phosphate; G6P = glucose 6-phosphate; PEP = phosphoenolpyruvate; R5P = ribose 5-phosphate; Ru5P = ribulose-5-phosphate; S7P = sedoheptulose 7-phosphate; X5P = xylulose-5-phosphate.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Expression of metabolites relating to glycolysis and the pentose phosphate pathway (PPP) in normal liver from control rats and tumors from N1S1 and McA-RH7777. Each graph shows the metabolite expression in normal liver (C_NL, left bar), McA-RH7777 (M_T, middle bar), and N1S1 (N_T, right bar). Error bars represent SD. 1,3-BPG = 1,3-bisphosphoglyceric acid; 3PG = 3-phosphoglyceric acid; 6-PG = 6-phosphogluconic acid; 6-PGL = 6-phosphogluconolactone; DHAP = dihydroxyacetone phosphate; E4P = erythrose 4-phosphate; F1,6BP = fructose 1,6-bisphosphate; F2,6BP = fructose 2,6-bisphosphate; F6P = fructose 6-phosphate; G3P = glyceraldehyde 3-phosphate; G6P = glucose 6-phosphate; PEP = phosphoenolpyruvate; R5P = ribose 5-phosphate; Ru5P = ribulose-5-phosphate; S7P = sedoheptulose 7-phosphate; X5P = xylulose-5-phosphate.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Control

Messenger RNA expression of glycolysis and pentose phosphate pathway (PPP)–related genes in N1S1 tumors, McA-RH7777 tumors, and normal liver. Glycolysis-related key enzymes include GLUT1, HK2, PFKFB3, PFK1, LDHA, and PKM. PPP-related key enzymes include G6PD, TALDO1, PGLS, PGD, RPIA, and TKT. Densitometry qualification of band intensity is presented as a percentage of relative densitometry normalized to the CYPA gene. Blue arrow, target protein. P values represent results from Mann–Whitney test, performed only after a significant difference across the three groups was found with a Kruskal–Wallis test. Error bars represent SD. C_NL = normal liver from control rats, M_T = tumor from McA-RH7777 rats, N_T = tumor from N1S1 rats.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Messenger RNA expression of glycolysis and pentose phosphate pathway (PPP)–related genes in N1S1 tumors, McA-RH7777 tumors, and normal liver. Glycolysis-related key enzymes include GLUT1, HK2, PFKFB3, PFK1, LDHA, and PKM. PPP-related key enzymes include G6PD, TALDO1, PGLS, PGD, RPIA, and TKT. Densitometry qualification of band intensity is presented as a percentage of relative densitometry normalized to the CYPA gene. Blue arrow, target protein. P values represent results from Mann–Whitney test, performed only after a significant difference across the three groups was found with a Kruskal–Wallis test. Error bars represent SD. C_NL = normal liver from control rats, M_T = tumor from McA-RH7777 rats, N_T = tumor from N1S1 rats.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: RNA Expression, MANN-WHITNEY, Control

Proliferation and metabolic alteration in radiofrequency ablation–stimulated N1S1 and McA-RH7777 tumors. (A) Increased Ki67 expression is observed in stimulated tumors relative to nonstimulated tumors. Scale bar, 50 µm. (B) The number of metastases in stimulated McA-RH7777 (S) is greater compared with nonstimulated tumors (NS). (C) 13C lactate-to-pyruvate ratio (LPR) is increased in N1S1 tumors from prestimulation to 3 days after treatment, but not in McA-RH7777 tumors. Error bars represent SD.

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Proliferation and metabolic alteration in radiofrequency ablation–stimulated N1S1 and McA-RH7777 tumors. (A) Increased Ki67 expression is observed in stimulated tumors relative to nonstimulated tumors. Scale bar, 50 µm. (B) The number of metastases in stimulated McA-RH7777 (S) is greater compared with nonstimulated tumors (NS). (C) 13C lactate-to-pyruvate ratio (LPR) is increased in N1S1 tumors from prestimulation to 3 days after treatment, but not in McA-RH7777 tumors. Error bars represent SD.

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing

Altered Metabolic Pathways between Stimulated Tumor and Nonstimulated Tumor in N1S1 and McA-RH7777 Models

Journal: Radiology: Imaging Cancer

Article Title: Characterizing Metabolic Heterogeneity of Hepatocellular Carcinoma with Hyperpolarized 13 C Pyruvate MRI and Mass Spectrometry

doi: 10.1148/rycan.230056

Figure Lengend Snippet: Altered Metabolic Pathways between Stimulated Tumor and Nonstimulated Tumor in N1S1 and McA-RH7777 Models

Article Snippet: Cell Lines Rat HCC cell lines N1S1 (CRL-1604, male) and McA-RH7777 (CRL-1601, male) were obtained from the American Type Culture Collection (ATCC).

Techniques:

Sorafenib demonstrates a marked induction of tumor cell death in both rat and human HCC cell lines. The data present statistical results regarding cell death rates for N1-S1 (A), McA-RH7777 (B), and HepG2 (C) in both the control group (vehicle) and the sorafenib treatment group. Additionally, NK cell lines exhibit a significant induction of death in HCC cell lines stronger than Sorafenib. The data provide statistical results for death rates of N1-S1 (A), McA-RH7777 (B), and HepG2 (C) in the control group (vehicle) and the NK cell line treatment group (****: P < 0.0001).

Journal: American Journal of Cancer Research

Article Title: Sorafenib plus memory like natural killer cell combination therapy in hepatocellular carcinoma

doi:

Figure Lengend Snippet: Sorafenib demonstrates a marked induction of tumor cell death in both rat and human HCC cell lines. The data present statistical results regarding cell death rates for N1-S1 (A), McA-RH7777 (B), and HepG2 (C) in both the control group (vehicle) and the sorafenib treatment group. Additionally, NK cell lines exhibit a significant induction of death in HCC cell lines stronger than Sorafenib. The data provide statistical results for death rates of N1-S1 (A), McA-RH7777 (B), and HepG2 (C) in the control group (vehicle) and the NK cell line treatment group (****: P < 0.0001).

Article Snippet: The HCC cell lines were cultured according to ATCC guidelines in which rat HCC cell lines (McA-RH7777 and N1-S1) were cultured in DMEM and IMDM (Gibco, Waltham, MA), and HepG2 cell line in EMEM (Corning, Manassas, VA).

Techniques: Control

The addition of IL-12 and IL-18 significantly enhances NK cell cytotoxicity against N1-S1 (A), McA-RH7777 (B), and HepG2 (C) HCC cells (*: P < 0.05, **: P < 0.01).

Journal: American Journal of Cancer Research

Article Title: Sorafenib plus memory like natural killer cell combination therapy in hepatocellular carcinoma

doi:

Figure Lengend Snippet: The addition of IL-12 and IL-18 significantly enhances NK cell cytotoxicity against N1-S1 (A), McA-RH7777 (B), and HepG2 (C) HCC cells (*: P < 0.05, **: P < 0.01).

Techniques:

The combination of sorafenib and NK cells significantly induces a higher death rate in N1-S1 (A), McA-RH7777 (B), and HepG2 (C) HCC cells compared to either treatment alone (**: P < 0.01). Furthermore, the cytotoxic function of sorafenib plus NK cell chemoimmunotherapy is enhanced through cytokine activation (Sorafenib plus ML NK), and the cell death ratio for (D) N1-S1, (E) McA-RH7777, and (F) HepG2 HCC cells significantly improve with this novel combination therapy (*: P < 0.05, **: P < 0.01).

Journal: American Journal of Cancer Research

Article Title: Sorafenib plus memory like natural killer cell combination therapy in hepatocellular carcinoma

doi:

Figure Lengend Snippet: The combination of sorafenib and NK cells significantly induces a higher death rate in N1-S1 (A), McA-RH7777 (B), and HepG2 (C) HCC cells compared to either treatment alone (**: P < 0.01). Furthermore, the cytotoxic function of sorafenib plus NK cell chemoimmunotherapy is enhanced through cytokine activation (Sorafenib plus ML NK), and the cell death ratio for (D) N1-S1, (E) McA-RH7777, and (F) HepG2 HCC cells significantly improve with this novel combination therapy (*: P < 0.05, **: P < 0.01).

Techniques: Activation Assay

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